Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Crescent Chemical Co Inc TRIS BUFFER PH 8.0 1 M SOLN
Tris buffer pH 8.0, 1 M solution molecular biology grade DNase, RNase, Protease not detected. The pH value of Tris buffer is temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per degree C decrease in temperature, and decreases 0.03 - 0.05 uni
Rockland Immunochemicals TM Buffer (50 mM Tris Cl, pH 7.5, 10 mM Magnesium Sulfate) - MB-059
1X TM Buffer (50 mM Tris HCl, 10 mM Magnesium Sulfate at pH 7.5) is suitable for molecular biology assays.
Boston Bioproducts Inc Tris-HCl Buffer (1 M, pH 8.0) - 500ml
The Tris-HCl Buffer is a widely used bufering reagent in molecular biology and biochemistry, acting to maintain a stable pH, and preserve the integrity of target macromolecules. The buffering capacity of Tris-HCl is effective in the pH range of 7.0 to 9.0, making it ideal for many biological applications like cell culture, molecular biology, DNA/RNA extraction, electrophoresis, enzyme assays and immunohistostaining.
Alkali Scientific CellPro™ TAE, 10X (Tris Acetate EDTA), 1L, 12/cs
Alkali Scientific™ CellPro™ TAE Tris Acetate EDTA is a fundamental reagent in nucleic acid electrophoresis, renowned for its exceptional buffering capacity and DNA solubilization properties. Designed to provide optimal performance and reproducibility, this solution enables precise separation and analysis of DNA fragments in agarose gel electrophoresis. Each batch undergoes rigorous sterile filtration to ensure purity and consistency, empowering researchers with confidence in their molecular biology experiments.
Teknova 1M Tris-HCl, pH 9.0. 1000mL, Sterile.
Tris (tris(hydroxymethyl)aminomethane) is used as a component ofbuffer solutions, especially for solutions ofnucleic acids. The useful buffer range for tris (pH 7–9) is similar to the pH of most living organisms. This, and its low cost, make tris one of the most common buffers in the biology/biochemistry laboratory.
Kd Medical Inc TRIS-GLYCINE-SDS BUFFER 10X
Tris-Glycine-SDS PAGE Buffer (10X) is a concentrated solution of Tris-Glycine-SDS in distilled/deionized water, especially intended for use as the running buffer in SDS-PAGE of proteins. One 1 liter bottle.
Biotang Inc Semi Dry Blot Transfer buffer(10X), 1000 ml
Semi Dry Blot Transfer buffer(10X), 1000 ml.
Alkali Scientific CellPro™ Tris-HCL Buffer,1M, pH 8.0, 1L, 12/cs
Alkali Scientific™ CellPro™ Tris solution offers sterile and filtered Tris buffer for various laboratory applications. This high-quality solution is meticulously prepared to ensure purity and consistency, meeting the stringent requirements of scientific research and experimentation. With its sterile formulation, it minimizes the risk of contamination, making it suitable for a wide range of cell culture and molecular biology procedures. The filtered nature of the solution ensures clarity and reliability in experimental results, providing researchers with confidence in their findings. Whether used in protein biochemistry, DNA electrophoresis, or other molecular biology techniques, CellPro™ Tris solution delivers reliable performance and consistent results, contributing to the success of laboratory workflows.
Bioworld STE Buffer (10X), 4 L
STE ReagentCommon reagent for molecular biology. Used in DNA extraction, DNA electrophoresis and many other lab protocols.DNAse and RNAse free, 10X solutionTechnical Information:1X concentration solution containsNaCl (100mM) Tris-HCl, pH 8.0 (10mM) EDTA, pH 8.0 (1mM)
Research Products International Corp TG 10X Solution, 2 L
10X Formulation:
Glycine: 1.92 M
pH: 8.5
Add methanol according to protocol
TARGETMOL CHEMICALS INC P62-mediated mitophagy inducer
Also available in 1 mL, 2 mg, 5 mg, 10 mg, 50 mg, 100 mg, 200 mg and bulk. Please contact Fisher for quotes. P62-mediated mitophagy inducer (PMI) is a regulator of mitophagy. Purity 99.35%
Bioworld Tris-Acetate-SDS Running Buffer (10X), 1 L
TRIS-Acetate-SDS Running Buffer (10X) All bioWORLD running buffers are prepared with high quality reagents. Technical Information:Dilute with deionized water to make 1X. 10X concentration1X contains: 50mM Tris base, 50mM Tricine, 3.5mM SDS pH 8.24