Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Sigma Aldrich Fine Chemicals Biosciences Tricine BioPerformance Certified, suitable for cell culture, >=99% (titration) | 5704-04-1 | MFCD00004277 | 25G
Tricine BioPerformance Certified, suitable for cell culture, >=99% (titration) | Purity: >=99% (titration) | Mol Wt: 179.17 | 5704-04-1 | MFCD00004277 | 25G
Kd Medical Inc TRIS HCL, 1M, PH 7.5, 500ML
Tris, Hydrochloride is commonly used as a buffer component for phenol extractions of DNA or RNA and a buffering component of separating and stacking gels in the characterization of protein products by SDS-PAGE. 1M solution, pH 7.5. One 500ml bottle.
Sigma Aldrich Fine Chemicals Biosciences Ethylenediaminetetraacetic Acid | 60-00-4 | MFCD00003541 | 100g
Ethylenediaminetetraacetic Acid | Purity: 98% | Mol Wt: 292.24 | 60-00-4 | MFCD00003541 | 100g
Sigma Aldrich Fine Chemicals Biosciences Trizma Base | 77-86-1 | MFCD00004679 | 100g
Trizma Base | Purity: 99% | Mol Wt: 121.14 | 77-86-1 | MFCD00004679 | 100g
Rockland Immunochemicals 1.0 M Tris HCl pH 8.5 - MB-027-1000
Tris solution is an abbreviation of the organic compound known as tris(hydroxymethyl)aminomethane. Tris is extensively used in biochemistry and molecular biology. In biochemistry, Tris is widely used as a component of additional buffer solutions, such as in TAE and TBE buffer, especially for solutions of nucleic acids. Visit our newly expanded web site at www.rockland-inc.com for methods using this and other buffers.
Teknova 10mM Tris-HCl, pH 8.0. 1000mL, DNase/RNase Tested, Sterile 6 pack.
Tris (tris(hydroxymethyl)aminomethane) is used as a component ofbuffer solutions, especially for solutions ofnucleic acids. The useful buffer range for tris (pH 7–9) is similar to the pH of most living organisms. This, and its low cost, make tris one of the most common buffers in the biology/biochemistry laboratory.
eMolecules 610769-36-3 | TAE buffer | MFCD00236357 | 25g
Combi-Blocks, Inc. | TAE buffer | 25g | 586078156 | QF-5924 | 95.000 | 610769-36-3 | MFCD00236357 | 473.432 | C16H31N3O13
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Boston Bioproducts Inc 10mM Tris-HCl pH 7.5 1L
Tris-HCl Buffer (10 mM pH 7 5) - 1L (For Research Use and Further Manufacturing Only)
eMolecules 2167889-81-6 | tert-butyl 4-(hydroxymethyl)-2,2-dimethyl-piperidine-1-carboxylate | MFCD30497054 | 1g
Medchem Express | NSC-70220 | 5mg | 495799510 | HY-101796 | 4551-00-2 | 325.367 | C22H15NO2
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Electron Microscopy Sciences Tris Buffered Saline, pH 7.4 500 ML
C₄H₁₁NO₃ F.W. 121.14
Our buffers are ideal for maintaining a constant pH during fixation and have an ionic constitution to prevent the extraction of cellular components or the deposition of precipitates of fixative chemicals. The buffers are prepared fresh for each order and conveniently packaged.
pH: 7.4
Sigma Aldrich Fine Chemicals Biosciences mPAGER 4-20 Bis-Tris Preca
mPAGE 4-20% Bis-Tris Precast Gel 10x8 cmUnless otherwise stated in our catalog or other company documentation accompanying the product(s) our products are intended for research use only and are not to be used for any other purpose which includes but is not limited to unauthorized commercial uses in vitro diagnostic uses ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Kd Medical Inc TBS, 20X, PH7.4, 2000ML
20X Tris Buffer Saline is ready for dilution to a 1X working solution. Used in various research applications. One 2 Liter cubitainer.