Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Sigma Aldrich Fine Chemicals Biosciences CHES BioUltra, >=99.5% (T) | 103-47-9 | MFCD00003835 | 10G
CHES BioUltra, >=99.5% (T) | Purity: >=99.5% (T) | Mol Wt: 207.29 | 103-47-9 | MFCD00003835 | 10G
Sigma Aldrich Fine Chemicals Biosciences CAPS BioXtra, >=99% | 1135-40-6 | MFCD00003837 | 100G
CAPS BioXtra, >=99% | Purity: >=99% | Mol Wt: 221.32 | 1135-40-6 | MFCD00003837 | 100G
Alkali Scientific CellPro™ Tris-HCL Buffer 1M, pH 7.5, 1L
Alkali Scientific™ CellPro™ Tris solution offers sterile and filtered Tris buffer for various laboratory applications. This high-quality solution is meticulously prepared to ensure purity and consistency, meeting the stringent requirements of scientific research and experimentation. With its sterile formulation, it minimizes the risk of contamination, making it suitable for a wide range of cell culture and molecular biology procedures. The filtered nature of the solution ensures clarity and reliability in experimental results, providing researchers with confidence in their findings. Whether used in protein biochemistry, DNA electrophoresis, or other molecular biology techniques, CellPro™ Tris solution delivers reliable performance and consistent results, contributing to the success of laboratory workflows.
Boston Bioproducts Inc HEPES Buffered Saline (2X) - 250ml
HEPES [2-(4-(2-hydroxyethyl) piperazin-1-yl)ethanesulfonic acid] Buffered Saline is a versatile buffer that maintains a stable pH in the range of 6.8 to 8.2, making it suitable for biological applications including in tissue culture, cell culture, cell isolation procedures, and for the preparation of plasmid DNA. It can also be used as a reagent for preparing transfection mixtures, and in protein isolation and activity studies. This buffer is made at 2X concentration.
Sigma Aldrich Fine Chemicals Biosciences CAPS | 1135-40-6 | MFCD00003837 | 250g
CAPS | Purity: 99% | Mol Wt: 221.32 | 1135-40-6 | MFCD00003837 | 250g
Sigma Aldrich Fine Chemicals Biosciences Trizma Base | 77-86-1 | MFCD00004679 | 100g
Trizma Base | Purity: 99% | Mol Wt: 121.14 | 77-86-1 | MFCD00004679 | 100g
Sigma Aldrich Fine Chemicals Biosciences CHES BioXtra, >=99.0% (titration) | 103-47-9 | MFCD00003835 | 25G
CHES BioXtra, >=99.0% (titration) | Purity: >=99.0% (titration) | Mol Wt: 207.29 | 103-47-9 | MFCD00003835 | 25G
Sigma Aldrich Fine Chemicals Biosciences Ethylenediaminetetraacetic acid BioUltra, >=99.0% (KT) | 60-00-4 | MFCD00003541 | 1KG
Ethylenediaminetetraacetic acid BioUltra, >=99.0% (KT) | Purity: >=99.0% (KT) | Mol Wt: 292.24 | 60-00-4 | MFCD00003541 | 1KG
Sigma Aldrich Fine Chemicals Biosciences Ethylenediaminetetraacetic Acid | 60-00-4 | MFCD00003541 | 100g
Ethylenediaminetetraacetic Acid | Purity: 98% | Mol Wt: 292.24 | 60-00-4 | MFCD00003541 | 100g
Sigma Aldrich Fine Chemicals Biosciences Ethylenediaminetetraacetic acid anhydrous, free-flowing, powder, Redi-Dri(TM), ACS reagent, 99.4-100.6% | 60-00-4 | MFCD00003541 | 1KG
Ethylenediaminetetraacetic acid anhydrous, free-flowing, powder, Redi-Dri(TM), ACS reagent, 99.4-100.6% | Purity: 99.4-100.6% | Mol Wt: 292.24 | 60-00-4 | MFCD00003541 | 1KG
Teknova DNA Suspension Buffer, RNAse DNAse Tested (10mM Tris, 0.1mM EDTA), pH 8.0. 500mL, Sterile 8 pack.
TE buffer is a salt solution that is commonly used to resuspend (or dilute) DNA and RNA. Composed of Tris base and EDTA, these molecules work together to maintain pH and prevent nucleic acids from degradation.
Boston Bioproducts Inc Phosphate Buffered Saline, PBS (10X, Low Endotoxin) - 250ml
Phosphate Buffered Saline PBS (10X Low Endotoxin) - 250ml (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc Sodium Phosphate Buffer (1 M, pH 7.2) - 50ml
Sodium Phosphate Buffer (1 M pH 7 2) - 50ml (For Research Use and Further Manufacturing Only)